Alpha-amylase variants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/28 (2006.01) C11D 3/386 (2006.01)

Patent

CA 2365438

The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha- amylase. The variant of the parent Termamyl-like alpha-amylase, comprised an alternation at one or more positions selected from the group of W13, G48, T49, S50, Q51, A52, D53, V54, G57, G107, G108, A111, S168 and M197.

La présente invention concerne une variante d'une alpha-amylase mère du type Termamyle, laquelle variante présente des propriétés modifiées, en particulier une capacité réduite de clivage d'un substrat à proximité d'un point de branchement, et une spécificité de substrat améliorée et/ou une activité spécifique améliorée vis-à-vis de l'alpha-amylase mère.

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