Alpha-amylase variants with altered properties

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/56 (2006.01) C11D 3/386 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/28 (2006.01) C12N 15/63 (2006.01) D06M 16/00 (2006.01)

Patent

CA 2571864

The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, in particular laundry and dish-wash applications.

La présente invention concerne des variants (mutants) de polypeptides, en particulier d'alpha-amylases de type Termamyl, lesdits variants présentant une activité alpha-amylase et présentant une modification au niveau d'au moins l'une des propriétés suivantes relativement auxdites alpha-amylases parentes : spécificité du substrat, liaison au substrat, motif de clivage du substrat, stabilité thermique, profil d'activité/pH, profil de stabilité/pH, stabilité vis-à-vis de l'oxydation, dépendance du Ca2+, activité spécifique, dans des applications de lavage de vaisselle et de blanchisserie particulières.

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