Alpha-galactosidase as food-grade genetic marker

Details Alpha-galactosidase as food-grade genetic marker Alpha-galactosidase as food-grade genetic marker

C - Chemistry, Metallurgy

12

N

C12N 9/40 (2006.01) A61K 38/47 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/56 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2441628

The invention relates to a 4007 bp DNA fragment from the strain Lactococcus raffinolactis ATCC 43920 containing two genes. The first gene (named aga) codes for an enzyme with an alpha-galactosidase activity. The second gene (named galR) codes for a transcriptional regulator which would act as a regulator of aga. When present in a lactic acid bacterium such as Lactococcus lactis, this DNA fragment can modify the sugar fermentation profile of the strain from melibiose-negative to melibiose-positive. The utilisation of a culture media containing melibiose as the sole carbon source and bromcresol purple as pH indicator allows the identification of the melibiose-fermenting bacteria as yellow colonies on a purple background.

L'invention concerne un fragment d'ADN bp 4007 issu de la souche Lactococcus raffinolactis ATCC 43920 contenant deux gènes. Le premier gène (appelé aga) code une enzyme avec une activité alpha-galactosidase. Le second gène (appelé galR) code un régulateur transcriptionnel qui agit en tant que régulateur d'aga. Lorsqu'il se trouve dans une bactérie lactique telle que la Lactococcus lactis, ce fragment d'ADN peut modifier le profile de fermentation du sucre de la souche de mélibiose-négatif en mélibiose-positif. L'utilisation d'un milieu de culture contenant du mélibiose en tant que seule source de carbone et du bromocrésol pourpre en tant qu'indicateur de pH permet l'identification de bactéries de fermentation de mélibiose en tant que colonies jaunes sur un fond pourpre.

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