.alpha.-isomaltosylglucosaccharide-forming enzyme, process...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/24 (2006.01) A21D 2/18 (2006.01) A23C 9/00 (2006.01) A23C 9/13 (2006.01) A23C 9/154 (2006.01) A23C 9/156 (2006.01) A23G 1/00 (2006.01) A23G 3/00 (2006.01) A23G 4/00 (2006.01) A23G 9/32 (2006.01) A23L 1/236 (2006.01) A23L 1/30 (2006.01) A23L 1/308 (2006.01) A23L 1/32 (2006.01) A23L 2/60 (2006.01) A61K 8/60 (2006.01) A61K 8/66 (2006.01) A61K 47/26 (2006.01) A61Q 11/00 (2006.01) A61Q 19/00 (2006.01) C07H 3/04 (2006.01) C07H 3/06 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2385465

.alpha.-Isomaltosylglucosaccharide synthase capable of transferring a-glucosyl from a saccharide, which carries an .alpha.-glucosyl bond as the binding manner at the nonreducing end and has a degree of glucose polymerization of at least 2, without substantially elevating the reducing ability to thereby form a saccharide which carries an .alpha.-1,6 glucosyl bond as the binding manner at the nonreducing end and an .alpha.-1,4 glucosyl bond as a binding manner at other than the nonreducing end and has a degree of glucose polymerization of at least 3; cyclic tetrasaccharides obtained by using this enzyme; carbohydrates containing the same; and use thereof.

L'invention concerne une synthèse d'.alpha.-isomaltosylglucosaccharide capable de transférer un .alpha.-glucosyl à partir d'un saccharide, portant une liaison .alpha.-1,4-glucosyl assurant la liaison au niveau d'une extrémité non-réductrice et possédant un degré de polymérisation du glucose d'au moins 2, sans élever sensiblement la capacité de réduction, en vue de former ainsi un saccharide portant une liaison .alpha.-1,6-glucosyl assurant la liaison au niveau de l'extrémité de non-réduction et une liaison .alpha.-1,4-glucosyl assurant la liaison au niveau d'une autre extrémité que l'extrémité de non-réduction et possédant un degré de polymérisation du glucose d'au moins 3. L'invention concerne également des tétrasacchardides cycliques obtenus au moyen de cette enzyme, des carbohydrates renfermant ceux-ci et une utilisation de ceux-ci.

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