Alpha-n-acetylgalactosaminidase from clostridium perfringens

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/26 (2006.01) A01N 1/00 (2006.01) C12N 9/40 (2006.01)

Patent

CA 2308347

An isolated and purified .alpha.-N-acetyl-D-galactosaminidase from Clostridium perfringens is disclosed. A method for purifying and isolating the .alpha.-N- acetylgalactosaminidase from Clostridium perfringens by removing neuramidases is disclosed. A process for using the .alpha.-N-acetylgalactosaminidase from Clostridium perfringens in altering type A blood cells to type O blood cells is disclosed. A process for altering cells expressing blood group A epitope by using .alpha.-N-acetylgalactosaminidase isolated from Clostridium perfringens in altering the cells expressing blood group A epitope to cells expressing blood group O epitope is disclosed.

La présente invention concerne une .alpha.-N-acétyl-D-galactosaminidase isolée et purifiée provenant de Clostridium perfringens, et elle se rapporte également à un procédé permettant de purifier et d'isoler l'.alpha.-N-acétylgalactosaminidase de Clostridium perfringens en enlevant les neuramidases. L'invention a trait à un procédé permettant d'utiliser l'.alpha.-N-acétylgalactosaminidase de Clostridium perfringens pour transformer les cellules sanguines de type A en cellules sanguines de type O. L'invention concerne enfin un procédé permettant de modifier les cellules exprimant l'épitope du groupe sanguin A en utilisant l'.alpha.-N-acétylgalactosaminidase isolée de Clostridium perfringens pour transformer les cellules exprimant l'épitope du groupe sanguin A en cellules exprimant l'épitope du groupe sanguin O.

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