Alphavirus and alphavirus replicon particle formulations and...

A - Human Necessities – 61 – K

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A61K 39/12 (2006.01) A61K 9/19 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2668417

Disclosed are methods for preparing dried (preferably lyophilized) preparations comprising a population of alphaviruses or alphavirus replicon particles, a sugar or polyol, a surfactant and a salt and preparations made by said methods, both in the dried form but also as liquids prior to drying or after reconstituting dried preparations. These preparations may further comprise a plasticizer and/or a bulking agent. These preparations are readily reconstituted, with little or no loss in infectivity of the viruses or replicon particles.

La présente invention concerne des procédés de production de préparations séchées (de préférence lyophilisées) comprenant une population d'alphavirus ou de particules de réplicon d'alphavirus, un sucre ou polyol, un tensioactif et un sel, et concerne également les préparations obtenues au moyen de ces procédés, non seulement sous la forme séchée mais aussi sous forme de liquides avant le séchage ou après la reconstitution de préparations séchées. Ces préparations peuvent également comprendre un plastifiant et/ou un agent gonflant. Ces préparations sont reconstituées facilement, avec peu ou pas de perte d'infectiosité des virus ou des particules de réplicon.

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