Alphavirus-based vectors for persistent infection

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/00 (2006.01) C07K 14/18 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) C12N 15/86 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2407050

Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein 2 gene which, when operably incorporated into an alphavirus replicon particle, eukaryotic layered vector initiation system, alphavirus vector construct or RNA vector replicon, provides a noncytopathic phenotype or confers the ability to establish persistent replication. Also disclosed are RNA vector replicons, alphavirus vector constructs, alphavirus replicon particles and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules, as well as methods of using such replicons, constructs, particles and eukaryotic layered vector initiation systems for expression of recombinant proteins.

L'invention concerne des molécules d'acides nucléiques isolés qui comprennent le gène de la protéine non structurelle 2 d'alphavirus qui, intégré de manière fonctionnelle dans une particule de réplicon d'alphavirus, un système d'initiation du vecteur eucaryote à plusieurs couches, une structure de vecteur d'alphavirus ou un réplicon de vecteur d'ARN, fournit un phénotype non cytopathique ou confère la capacité de mettre en oeuvre une réplication durable. L'invention concerne aussi des réplicons de vecteur d'ARN, des structures de vecteur d'alphavirus, des particules de réplicon d'alphavirus et des systèmes d'initiation du vecteur eucaryote à plusieurs couches qui contiennent des molécules des acides nucléiques identifiés ci-dessus ainsi que des procédés pour utiliser ces réplicons, structures, particules et systèmes d'initiation du vecteur eucaryote à plusieurs couches à des fins d'expression de protéines recombinantes.

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