Alteration and modulation of protein activity by varying...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/42 (2006.01) C12N 9/00 (2006.01) C12N 9/24 (2006.01) C12N 15/00 (2006.01) C12P 21/00 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2752175

Embodiments of the invention include methods of altering the enzymatic activity or solubility of an extremophilic enzyme or post-translationally modifying a protein of interest via using isolated or partially purified glycosyltransferases and/or post-translational modification proteins, extracts of cells comprising glycosyltransferases and/or post-translational modification proteins, and/or in cells comprising one or more glycosyltransferases and/or post-translational modification proteins.

Les modes de réalisation selon l'invention comprennent des procédés pour altérer l'activité enzymatique ou la solubilité d'une enzyme extrêmophile ou pour modifier après traduction une protéine d'intérêt au moyen de glycosyltransférases isolées ou partiellement purifiées et/ou de protéines de modification post-traductionnelles, d'extraits de cellules comprenant des glycosyltransférases et/ou des protéines de modification post-traductionnelles et/ou dans des cellules comprenant une ou plusieurs glycosyltransférases et/ou protéines de modification post-traductionnelles.

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