Alteration of amino acid compositions in seeds

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 1/22 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2220959

The present invention provides methods for increasing the level of preselected amino acids in seeds of plants, thereby enhancing the nutritional value of the seeds, by genetic modification. The present invention is particularly useful in increasing the methionine, lysine, and/or cysteine content in seeds of plants. Also provided, are isolated endogenous DNA molecules which encode soybean albumins. The present invention also provides an antibody which is capable of specifically binding to soybean albumins. The present invention further provides methods for isolating and purifying 2S albumins.

La présente invention se rapporte à des procédés permettant d'accroître la teneur en acides aminés sélectionnés dans des graines de plantes, par modification génétique. La présente invention est particulièrement utile s'agissant d'accroître la teneur en méthionine, en lysine et/ou en cystéine dans des graines de plantes. L'invention concerne également des molécules d'ADN endogènes isolées qui codent des albumines du soja. La présente invention concerne également un anticorps qui peut se lier spécifiquement aux albumines du soja. L'invention concerne en outre des procédés permettant d'isoler et de purifier des albumines 2S.

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