Altered protein expression in hypoxic trophoblasts

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/47 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 14/575 (2006.01) C07K 14/59 (2006.01) C07K 14/75 (2006.01) C07K 14/765 (2006.01) C07K 14/775 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2218535

This invention is the discovery that hypoxic mammalian fetal trophoblast cells are a valid model of abnormal maternal plancental function and of other invasive cell types. The trophoblast cells differentially express a number of proteins when grown under hypoxic conditions. These proteins are useful for determining the health of the trophoblasts, the condition of a maternal placental interface and the invasive properties of metastatic and other invasive cell types. The hypoxic cytotrophoblast cells provide a convenient means to isolate these proteins and develope assay kits based upon the presence or absence of the proteins in a human test sample.

Cette invention concerne une découverte selon laquelle les cellules de trophoblastes foetales hypoxiques chez les mammifères sont un modèle valide d'une fonction placentaire maternelle anormale ainsi que d'autres types de cellules invasives. Les cellules de trophoblastes expriment de manière différente un certain nombre de protéines lorsqu'elles sont cultivées dans des conditions hypoxiques. Ces protéines peuvent être utiles afin de déterminer l'état de santé des trophoblastes, l'état d'une interface placentaire maternelle, ainsi que les propriétés invasives de cellules de type métastatique ou d'autres types de cellules invasives. Les cellules de cyclotrophoblastes hypoxiques constituent un moyen pratique d'isoler ces protéines et d'élaborer des nécessaires de dosage qui s'appuient sur la présence ou l'absence de ces protéines dans un échantillon provenant d'un être humain.

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