Altered recombinases for genome modification

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 9/00 (2006.01) C12N 11/04 (2006.01) C12N 15/74 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2400087

The present invention describes methods of identifying altered recombinases and compositions thereof, wherein at least one amino acid is different from a parent, wild-type recombinase and the altered recombinase has improved recombination efficiency towards wild-type and/or pseudo at site sequences relative to the parent, wild-type recombinase. The present invention also includes methods of modifying the genomes of cells using the altered recombinases, including methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell.

La présente invention concerne des procédés permettant d'identifier des recombinases modifiées et des compositions de celles-ci, dans lesquelles au moins un acide aminé est différent d'une recombinase mère du type sauvages et la recombinase modifiée possédant une efficacité de recombinaison améliorée par rapport aux séquence du type sauvage et/ou de site aléatoire d'attachement relatives à la recombinase mère du type sauvage. La présente invention concerne également des procédés permettant de modifier les génomes des cellules mettant en oeuvre les recombinases modifiées, notamment des procédés d'intégration spécifique de site d'une séquence polynucléotidique étudiée dans un génome d'une cellule eucaryote.

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