Alternative compositions and methods for the culture of stem...

Details Alternative compositions and methods for the culture of stem... Alternative compositions and methods for the culture of stem...

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/0735 (2010.01) A61K 35/12 (2006.01)

Patent

CA 2456981

Methods and cell culture medium for the generation of human pluripotent embryonic stem cells are disclosed. Human embryonic stem cells are cultured with human granulosa feeder cells, muscle cells, Fallopian ductal epithelial cells, bone marrow stromal cells, and skin fibroblasts and the embryonic stem cells maintain their pluripotent phenotype. The human pluripotent embryonic stem cells can be cultured without feeder cells, and in the presence of supplemental growth factors. The human pluripotent embryonic stem cells can be alternatively cultured with conditioned medium obtained from a cell culture capable of maintaining human embryonic stem cells in a pluripotent state, wherein the cell culture is a human granulosa cell culture.

L'invention concerne des procédés et un milieu de culture cellulaire pour produire des cellules souches embryonnaires humaines multipotentes. Les cellules souches embryonnaires humaines sont mises en culture avec des cellules nourricières de la granulosa humaine, des cellules musculaires, des cellules épithéliales des trompes de Fallope, des cellules du stroma de la moelle osseuse, et des fibroblastes cutanés et les cellules souches embryonnaires conservent leur phénotype multipotent. Les cellules souches embryonnaires humaines multipotentes peuvent être mises en culture sans cellules nourricières et en présence de facteurs de croissance supplémentaires. Les cellules souches embryonnaires humaines multipotentes peuvent également être mises en culture avec un milieu conditionné provenant d'une culture cellulaire apte à conserver les cellules souches embryonnaires humaines dans un état multipotent, la culture cellulaire étant une culture de cellules de granulosa humaine.

Affiliated with

Also associated with

Rating

Alternative compositions and methods for the culture of stem... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Alternative compositions and methods for the culture of stem..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alternative compositions and methods for the culture of stem... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1519419