Alternatively spliced nucleic acid molecules

Details Alternatively spliced nucleic acid molecules Alternatively spliced nucleic acid molecules

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) C07K 14/52 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2483431

The invention provides an isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence of at least 1877 contiguous nucleotides of (a) SEQ ID NO: 1 or (b) a nucleotide sequence encoding an angiogenic factor, wherein the nucleotide sequence comprises cDNA comprising exons 1-5 of a vascular endothelial growth factor (VEGF) joined to genomic DNA comprising introns 5, 6, and 7 and exons 6, 7, and 8 of VEGF and further comprising a mutation in one or more of the splice donor, branch point, and splice acceptor regions contained in an exon selected from the group consisting of exons 5, 6, 7, and 8, wherein the mutation promotes the production of one isoform of VEGF as compared to another isoform of VEGF. Expression constructs, compositions, and cells comprising such a nucleic acid molecule also are provided by the invention.

L'invention concerne une molécule, isolée ou purifiée, d'acides nucléiques essentiellement constituée d'une séquence nucléotidique d'au moins 1877 nucléotides contigus de (a) SEQ ID NO: 1 ou (b) de séquence nucléotidique codant pour un facteur angiogénique, la séquence nucléotidique comprenant un ADNc comportant les exons 1-5 d'un facteur de croissance endothéliale vasculaire (VEGF) joints à de l'ADN génomique comportant les introns 5, 6, et 7 et les exons 6, 7 et 8 du VEGF et comportant aussi une mutation dans un ou plusieurs sites de donneur d'épissage, de point de branchement et d'accepteur d'épissage contenus dans un exon sélectionné dans le groupe comprenant les exons 5, 6, 7 et 8, la mutation favorisant la production d'une isoforme de VEGF par comparaison à une autre isoforme de VEGF. L'invention concerne aussi des constructions, des compositions et des cellules contenant une telle molécule d'acides nucléiques.

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