Amino acid modified polypeptides

Details Amino acid modified polypeptides Amino acid modified polypeptides

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C07K 14/00 (2006.01) C12N 1/00 (2006.01) C12N 1/14 (2006.01) C12N 1/19 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2328423

Incorporation of certain amino acid analogs into polypeptides produced by cells which do not ordinarily provide polypeptides containing such amino acid analogs is accomplished by subjecting the cells to growth media containing such amino acid analogs. The degree of incorporation can be regulated by adjusting the concentration of amino acid analogs in the media and/or by adjusting osmolality of the media. Such incorporation allows the chemical and physical characteristics of polypeptides to be altered and studied.

On incorpore certains analogues d'acides aminés dans des polypeptides produits par des cellules ne produisant ordinairement pas de polypeptides contenant lesdits analogues, en exposant les cellules à des milieux de culture contenant lesdits analogues d'acides aminés. Le degré d'incorporation peut être régulé par modulation de la concentration d'analogues d'acides aminés dans les milieux et/ou par l'ajustement de l'osmolalité des milieux. Ladite incorporation permet de modifier et d'étudier les caractéristiques chimiques et physiques des polypeptides.

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