Amino acid sequences directed against cxcr4 and other gpcrs...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/28 (2006.01) A61K 39/395 (2006.01) A61P 31/18 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2724208

The present invention relates to amino acid sequences that are directed against (as defined herein) G-protein coupled receptors (GPCRs) and in particular to CXCR4 and CXCR7, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences (also referred to herein as "amino acid sequences of the invention", "compounds of the invention", and "polypeptides of the invention", respectively). Furthermore, the invention provides a new method of making amino acid sequences that are directed against transmembrane protein, and in particular for multiple spanning transmembrane proteins for which the native conformation cannot be reproduced in other "in vitro" system (e.g. GPCRs in general).

L'invention porte sur des séquences d'acides aminés qui sont dirigées contre (tels que définis ci-après) les récepteurs couplés à la protéine G (GPCR) et, en particulier, contre CXCR4 et CXCR7, et sur des composés ou des produits de synthèse, en particulier des protéines et des polypeptides, qui comprennent ou qui sont essentiellement composés d'une ou plusieurs desdites séquences d'acides aminés (également dénommées dans la description "séquences d'acides aminés selon l'invention", "composés selon l'invention" et "polypeptides selon l'invention", respectivement). L'invention se rapporte en outre à un nouveau procédé de fabrication de séquences d'acides aminés qui sont dirigées contre la protéine transmembranaire, et en particulier les protéines transmembranaires traversant la membrane plusieurs fois, pour lesquelles on ne peut pas reproduire la forme native dans un autre système "in vitro" (p.ex. les GPCR en général).

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