Amino acid substituted 4-aminophenazones for detectjion of...

Details Amino acid substituted 4-aminophenazones for detectjion of... Amino acid substituted 4-aminophenazones for detectjion of...

C - Chemistry, Metallurgy

12

Q

150/11, 150/15,

C12Q 1/48 (2006.01) C07K 5/02 (2006.01) C07K 5/06 (2006.01) C07K 5/072 (2006.01) C12Q 1/26 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 1340131

Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilirubin oxidase.

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