Amino polyol amine oxidase polynucleotides and related...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01N 63/02 (2006.01) A23K 1/00 (2006.01) A23K 1/165 (2006.01) A23K 3/03 (2006.01) C12N 9/06 (2006.01) C12N 9/16 (2006.01) C12N 15/82 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2332722

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

L'invention porte sur des polynucléotides et les polypeptides associés de l'enzyme APAO isolés à partir de l'Exophiala spinifera. Les polynucléotides codant pour l'enzyme APAO peuvent servir à transformer des cellules de plantes susceptibles d'être infectées par le Fusarium ou d'autres mycoses productrices de toxines. Les plantes peuvent être régénérées à l'aide de cellules végétales transformées. L'invention porte également sur l'expression de l'APAO et de l'estérase de fumonisine dans des plantes transgéniques. On peut ainsi produire une plante transgénique capable de dégrader la fumonisine et de produire les enzymes de dégradation. L'invention porte en outre sur des procédés de production d'enzymes de dégradation dans des systèmes procariotes et eucariotes non végétaux. L'invention porte de plus sur des procédés de détoxification des graines, de traitement des graines, d'ensilage, de récoltes d'aliments, et de détoxification des microbes de la panse et des aliments pour animaux.

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