Amino polyol amine oxidase polynucleotides and related...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01N 63/02 (2006.01) A23K 1/00 (2006.01) A23K 1/165 (2006.01) A23K 3/03 (2006.01) C12N 9/06 (2006.01) C12N 9/16 (2006.01) C12N 15/82 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2333085

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

Polynucléotides et polypeptides associés de l'enzyme aminopolyolamineoxydase (APAO) isolée de Exophiala spinifera et de Rhinocladiella atrovirens. Les polynucléotides codant l'enzyme APAO peuvent être utilisés pour transformer des cellules végétales normalement sensibles à Fusarium et à d'autres infections fongiques produisant des toxines. Des plantes peuvent être régénérées à l'aide desdites cellules végétales transformées. La présente invention concerne en outre l'expression de l'APAO et d'une fumosine estérase dans une plante transgénique. Il est ainsi possible de produire une plante transgénique ayant la capacité de dégrader la fumosine, ainsi que la capacité de produire les enzymes dégradantes. Elle concerne encore des procédés de production de l'enzyme APAO dans des systèmes tant procaryotes qu'eucaryotes non végétaux. Des procédés de détoxication du grain, du traitement du grain, de l'ensilage, des denrées vivrières et de l'alimentation animale et des bactéries du rumen sont également décrits.

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