Aminopeptidase

Details Aminopeptidase Aminopeptidase

C - Chemistry, Metallurgy

12

N

C12N 9/48 (2006.01) C12N 9/54 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2460309

A gram-negative bacterial cell is described that is deficient in a chromosomal gene present in a wild~-type such cell which gene shares at least 80% sequence identity with the native sequence of the yfcK gene and encodes an aminopeptidase. Alternatively, a gram-negative bacterial cell is deficient in a chromosomal gene present in a wild-type such cell which gene encodes an aminopeptidase that shares at least 80% sequence identity with the native sequence of aminopeptidase b2324. Either of these types of cells, when 10 comprising a nucleic acid encoding a heterologous polypeptide, produces an N- terminal unclipped polypeptide when it is cultured and the polypeptide recovered, with virtually no N-terminal clipped polypeptide produced as an impurity. Conversely, a method is provided for cleaving an N-terminal amino acid from a polypeptide comprising contacting the polypeptide with an aminopeptidase sharing at least 80% sequence identity with the native sequence of aminopeptidase b2324.

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