Ammonia-oxidizing bacteria and methods of using and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/11 (2006.01) C02F 3/34 (2006.01) C07H 21/00 (2006.01) C07K 14/195 (2006.01) C12N 1/20 (2006.01) C12N 11/00 (2006.01) C12P 3/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2499482

Described herein are novel ammonia-oxidizing bacteria as well as isolated nucleotide sequences representative of 16S rDNA of these ammonia-oxidizing bacteria. Particular bacteria of the present invention are tolerant of freshwater environments, saltwater environments or both. Furthermore, in various embodiments, various bacteria of the present invention are capable of surviving a freeze-drying process, and may remain viable thereafter. Compositions including various combinations of the bacteria are further described, as are polymerase chain reaction (PCR) primers and oligonucleotide probes that may be used to detect these bacteria based on their 16S rDNA. Methods for preventing or alleviating the accumulation of ammonia in aqueous environments, such as aquaria and wastewater are also provided, using the ammonia-oxidizing bacteria of the present invention. Methods for detecting the bacteria of the present invention are also provided. Such methods may be effected by any conventional methology, such as with a DNA chip.

L'invention concerne de nouvelles bactéries qui oxydent l'ammoniac ainsi que des séquences de nucléotides isolées représentant de l'ADNr 16S des bactéries oxydant l'ammoniac. Les bactéries particulières de l'invention sont tolérantes dans des environnements d'eau fraîche, des environnements d'eaux salées ou les deux. Dans de nombreux modes de réalisation, de nombreuses bactéries sont, de plus, capables de survivre à un processus de lyophylisation, et peuvent rester en vie à la suite de ce processus. L'invention concerne des compositions comprenant de nombreuses combinaisons des bactéries, telles que des amorces par PCR et des sondes d'oligonucléotides qui peuvent être utilisées pour détecter lesdites bactéries sur la base de leur ADNr 16S. L'invention concerne également des méthodes de prévention ou d'atténuation de l'accumulation d'ammoniac dans des environnements aqueux, tels que dans les aquariums et les eaux usées, utilisant lesdites bactéries oxydant l'ammoniac. L'invention concerne également des procédés de détection desdites bactéries, ces procédés pouvant être réalisés au moyen d'une méthodologie classique, par exemple au moyen d'une puce d'ADN.

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