Ammonium sulfate for neutralization of inhibitory effects

Details Ammonium sulfate for neutralization of inhibitory effects Ammonium sulfate for neutralization of inhibitory effects

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2433542

Ribonucleic acid (RNA) is a substance synthesized biologically and synthetically. RNA serves many functions as information molecules, reaction substrate, reaction catalyst, recognition element, structural element etc. For most analysis methods and functions concerning RNA itself or RNA as an analysis tool the purity of RNA is important. That means that e.g., other molecules which bind the RNA are able to inhibit the analysis/function of/using the RNA molecule or destroy the structure of the RNA molecule. For this reason it is important to eliminate the inhibitory or destroying function of molecules binding to the RNA. Moreover stable secondary structures of RNA often results in reduced function and analysis quality. Secondary structure depends on the environment of the RNA molecule. The present invention features the addition of ammonium sulfate (NH4)2SO4 to RNA in solution to eliminate the inhibitory or destroying effect of molecules binding to the RNA to be taken as information molecule, reaction substrate, reaction catalyst, recognition element, structural element etc. Furthermore (NH4)2SO4 solves secondary structures of RNA to make RNA more accessible to reactions and analysis.

L'acide ribonucléique (ARN) est une substance synthétisée de manière biologique et synthétique et remplit plusieurs fonctions telle que la molécule d'information, le substrat de réaction, le catalyseur de réaction, l'élément de reconnaissance, l'élément structurel, etc. Pour la plupart des méthodes d'analyse et des fonctions concernant l'ARN en soi ou l'ARN en tant qu'instrument d'analyse, la pureté de l'ARN est importante. Ce qui signifie par ex., que d'autres molécules qui se fixent sur l'ARN, peuvent inhiber l'analyse/la fonction de/d'utilisation de la molécule d'ARN ou détruire la structure de la molécule d'ARN. C'est la raison pour laquelle il est important d'éliminer la fonction inhibitrice ou destructrice de molécules se fixant sur l'ARN. En outre, des structures secondaires stables d'ARN entraînent fréquemment une qualité de fonction et d'analyse réduite. La structure secondaire dépend de l'environnement de la molécule d'ARN. L'invention souligne l'adjonction de sulfate d'ammonium (NH¿4?)2SO¿4? à l'ARN en solution afin d'éliminer l'effet inhibiteur ou destructeur de molécules se fixant à l'ARN à considérer comme molécule d'information, de substrat de réaction, de catalyseur de réaction, d'élément de reconnaissance, d'élément structural, etc. Le (NH¿4?)2SO¿4? dissout qui plus est des structures secondaires d'ARN afin de rendre l'ARN plus accessible aux réactions et aux analyses.

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