Amperometric sensor and method for its manufacturing

Details Amperometric sensor and method for its manufacturing Amperometric sensor and method for its manufacturing

G - Physics

01

N

G01N 33/487 (2006.01) G01N 27/49 (2006.01)

Patent

CA 2654220

The invention refers to an amperometric sensor (1) configured for implantation into the living body of a human or animal to measure the concentration of an analyte in a body fluid, said sensor (1) comprising a counter electrode (2) and a working electrode (3), said working electrode (3) comprising a sensing layer (9) which is permeable for water and arranged on a support member (5) adjacent to a contact pad (7), said sensing layer (9) comprising an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal, the sensing layer (9) having an upper surface facing the body fluid and a lower surface facing away from the body fluid. According to the invention the immobilized enzyme is distributed in the sensing layer (9) in such a way that the enzyme concentration in the middle between its upper and lower surface is at least as high as on the upper surface of the sensing layer (9).

La présente invention concerne un capteur ampérométrique (1) configuré pour être implanté dans le corps vivant d'un être humain ou d'un animal afin de mesurer la concentration d'un analyte dans un fluide corporel, le capteur (1) comprenant une contre-électrode (2) et une électrode de travail (3), celle-ci (3) comprenant une couche de captage (9) perméable à l'eau et placée sur un élément de soutien (5) adjacent à un tampon de contact (7), la couche de captage (9) comprenant une enzyme immobilisée, capable d'agir de manière catalytique en présence de l'analyte pour entraîner un signal électrique, la couche de captage (9) ayant une surface supérieure faisant face au fluide corporel et une surface inférieure ne faisant pas face au fluide corporelle. Selon l'invention, l'enzyme immobilisée est distribuée dans la couche de captage (9) de manière à ce que la concentration en enzymes au milieu, entre sa surface supérieure et sa surface inférieure soit au moins aussi élevée que sur la surface supérieure de la couche de captage (9).

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