Amphotropic retrovirus packaging cell line, process for its...

Details Amphotropic retrovirus packaging cell line, process for its... Amphotropic retrovirus packaging cell line, process for its...

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) C12N 5/10 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2331440

A titre of retroviral vectors of at least about 107 colony-forming units/ml cell culture supernatants is obtained using an amphotropic retroviral packaging cell line which contains functional gag, pol and env genes integrated into the genome in which the expression of the genes gag and pol is regulated independently of the expression of the env genes, wherein the genome of the said cell line contains one or two functionally active env genes.

Un titre de vecteurs rétroviraux d'au moins environ 10?7¿ unités formant des colonies par ml de surnageant de culture cellulaire est obtenu à l'aide d'une lignée cellulaire d'encapsidation rétrovirale amphotropique qui contient des gènes fonctionnels gag, pol et env intégrés dans le génome dans lequel l'expression des gènes gag et pol est régulée indépendamment de l'expression des gènes env, le génome de ladite lignée cellulaire contenant un ou deux gènes env fonctionnellement actifs.

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