Amplification method for a polynucleotide

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) G01N 15/06 (2006.01) G01N 33/00 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2310564

The nucleic acid amplification procedures of the present invention provide methods for amplification of a nucleic acid comprised of a first strand, comprising (a) using the first strand to generate copies of a second strand at a first location, (b) moving the copies of the second strand to a second location, and (c) using the copies of the second strand to generate copies of at least a portion of the first strand. Target nucleic acids used in the context of the present method include RNA or DNA, either single stranded or double stranded, using primer extension or joining-type protocols. Embodiments are set forth for automated forms of the claimed procedures.

L'invention concerne des procédés d'amplification d'acides nucléiques permettant d'amplifier un acide nucléique composé d'un premier brin, ce qui consiste à (a) utiliser ce premier brin afin de générer des copies d'un deuxième brin à un premier emplacement, (b) déplacer les copies du deuxième brin vers un deuxième emplacement et (c) utiliser les copies du deuxième brin afin de générer des copies d'au moins une partie du premier brin. Ce procédé met en application ARN ou ADN, soit simple brin, soit double brin, en tant qu'acides nucléiques ciblés et utilise des protocoles d'extension d'amorce ou de liaison. L'invention présente des modes de réalisation automatiques de ces procédés.

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