C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
195/1.12, 150/8.
C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2034883
63542-2400 Abstract The invention provides a method for amplification of nucleotide sequences. The nucleic acid fragment to be amplified comprises an unknown sequence, a first priming region of known nucleotide sequence for hybridisation with a first fragment and a vectorette portion at least one fragement of which has a nucleic acid sequence which in either its double stranded complementary form or its single stranded form is capable of being bound by protein. The target nucleic acid fragment/vectorette unit is treated with appropriate nucleoside triphosphates and an agent for polymerisation of the nucleoside triphosphates under hybridising conditions. Under these conditions an extension product of a first priming region is synthesised complementary to a single stranded target nucleic acid/vectorette unit having a first priming region to which is hybridised a first primer selected so as to be substantially complementary to the first priming region. No such extension product is synthesised complementary to single stranded target nucleic acid fragment/vectorette units having no such first priming region. The method is particularly useful for the amplification of sequences only a portion of which is known and enables long nucleotide sequences to be rapidly and efficiently sequenced.
Anwar Rashida
Copley Clive G.
Markham Alexander F.
Mcpheat William L.
Smith John C.
Smart & Biggar
Zeneca Limited
LandOfFree
Amplification methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Amplification methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Amplification methods will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1542033