Amplification of dna to produce single-stranded product of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/00 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2483349

The present disclosure relates to methods for generating single-stranded DNA molecules of defined sequence and length. Specifically, a region of template containing target sequence is amplified by PCR or RCA, exogenous sequence is introduced by primers or probes used in amplification, double-stranded amplification products are converted to single-stranded amplification products, and single-stranded amplification products are trimmed to produce short single-stranded DNA molecules of defined sequence and length.

la présente invention concerne des techniques permettant de produire des molécules d'ADN à simple brin de séquence et de longueur définies. Spécifiquement, on amplifie une région de matrice par réaction en chaîne de la polymérase (PCR) ou par cercle roulant (RCA), on introduit une séquence exogène au moyen d'amorces ou de sondes utilisées pour l'amplificatio et l'on transforme des produits d'amplification à double brin en produits d'amplification à simple brin. Une fois rognés, ces produits d'amplification à simple brin produisent des molécules d'ADN courtes à simple brin de séquence et de longueur définies.

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