Amplification of hiv-1 sequences for detection of sequences...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2419649

Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using theamplification oligonucleotides specific forgag and pol target sequences are disclosed.

L'invention concerne des séquences oligonucléotidiques d'acides nucléiques permettant, d'une part, d'amplifier différentes portions des gènes <i>gag</i> et <i>pol</i> du VIH-1 et, d'autre part de détecter ces séquences d'acides nucléiques amplifiées. L'invention concerne également des méthodes permettant d'amplifier et de détecter un acide nucléique VIH-1 dans un prélèvement biologique par amplification d'oligonucléotides propres aux séquences cibles <i>gag</i> et <i>pol</i>.

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