Amplification of response from expressed recombinant protein

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 19/00 (2006.01) A61K 31/70 (2006.01) A61K 39/00 (2006.01) A61K 39/12 (2006.01) A61K 39/385 (2006.01) A61K 48/00 (2006.01) C07K 14/005 (2006.01) C12N 9/96 (2006.01)

Patent

CA 2244881

The present invention relates to a method for amplifying the response from a protein expressed from a recombinant vaccinia vector. The present invention may be used to amplify the intended effect of expressed proteins in any method where such expression is desired, for example, in vaccine production, vaccination and gene therapy. The present invention also relates to synthetic peptides, nucleic acid sequences, preferably DNA sequences and constructs comprising a vector linked to a DNA segment which encodes a protein containing a signal peptide at its N-terminus and an anchor sequence at its C-terminus. These expression vectors are generally useful for expressing polypeptides in a variety of animal cells.

L'invention concerne un procédé d'amplification de la réponse d'une protéine exprimée par un vecteur de recombinaison vaccinal. L'invention peut être utilisée pour amplifier l'effet souhaité de protéines exprimées dans une méthode quelconque où une telle expression est souhaitée, par exemple dans la production d'un vaccin, la vaccination et la thérapie génique. L'invention concerne, également, des peptides de synthèse, des séquences d'acides nucléiques, de préférence des séquences d'ADN et des produits d'assemblage comprenant un vecteur lié à un segment d'ADN qui code pour une protéine contenant un peptide signal à son extrémité N-terminale et une séquence d'ancrage à son extrémité C-terminale. Ces vecteurs d'expression sont généralement utiles pour exprimer des polypeptides, dans différentes cellules animales.

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