C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) A61K 35/14 (2006.01) C12N 5/08 (2006.01)
Patent
CA 2462404
The present invention relates to a method of ex vivo amplification of neonatal T cells from umbilical cord blood which comprises obtaining light density mononuclear cells from a sample of umbilical cord blood and then culturing said light density mononuclear cells in a serum-deprived culture medium supplemented with various cytokine combinations. Particularly, there are reported the effects exerted by cytokine combinations including stem cell factor (SCF), interleukin-7, interleukin-4 and interleukin-2, on the amplification of T cells from cord blood mononuclear cells cultured for 10-11 days under serum-deprived conditions. Of all the combinations investigated, SCF plus interleukin-7 sustained the best fold increase (FI) of total nucleated cells (FI=6.4~1.17), amplifying preferentially CD4+ over CD8+ T cell subsets (FI=4.72~0.79 vs 2.73~1.2, respectively, p<0.05). The addition of interleukin-2 to this combination did not significantly increase the total number of cells generated (FI=7.4~2.27) but allowed preferential amplification of CD8+ over CD4+ T cells (FI=6.04~0.14 vs 1.67~0.6, respectively, p<0.05). Single strand conformation polymorphism analysis of the T-cell receptor V.beta.-chain rearrangements expressed by the expanded T cells indicated that the complexity of the T-cell repertoire had increased after 10 days of culture in the presence of SCF and IL-7. Interestingly, a modest expansion (FI=8.67~1.5) of myeloid progenitor cells was also observed in these cultures. These results indicate that it is possible, by modulating the cytokines added to the cultures, to expand specific T cell subsets for adoptive immunotherapy without lousing myeloid progenitor cells necessary for neutrophil recovery after cord blood transplantation.
Alfani Elena
Franco Migliaccio Annarita
Migliaccio Giovanni
Sanchez Massimo
Istituto Superiore Di Sanita
Macrae & Co.
LandOfFree
Amplification of t cells from human cord blood in... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Amplification of t cells from human cord blood in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Amplification of t cells from human cord blood in... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1996176