Amplification vectors based on trans-splicing

Details Amplification vectors based on trans-splicing Amplification vectors based on trans-splicing

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2479984

This invention provides process of amplification and/or expression of a sequence of interest in a cell by providing for generating within said cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby said amplicon is capable of amplifying in said cell and capable of expressing a sequence of interest.

La présente invention concerne un procédé d'amplification et/ou d'expression d'une séquence étudiée dans une cellule en réalisant la génération à l'intérieur de cette cellule au moins un amplicon par transépissage entre une séquence d'ARN conçu pour être capable de transépissage et un ARN cible, ce qui fait que ledit amplicon est capable, d'une part de s'amplifier dans ladite cellule, et d'autre part d'exprimer une séquence étudiée.

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