Amplified expression of dna sequences

Details Amplified expression of dna sequences Amplified expression of dna sequences

C - Chemistry, Metallurgy

12

N

195/1.21, 195/1.

C12N 15/00 (2006.01) C07K 14/02 (2006.01) C07K 14/09 (2006.01) C12N 1/38 (2006.01) C12N 15/69 (2006.01)

Patent

CA 1194435

ABSTRACT A process for the amplified expression of DNA sequences and for the improved production of amino acids, polypeptides and proteins coded for by those DNA sequences. The process comprises the steps of increasing the copy number per cell of a multicopy plasmid-carried DNA sequence coding for a desired product; combining culture media with a culture of those increased copy number cells; culturing the resulting culture under conditions appropriate for expression of the plasmid-carried DNA sequence; and harvesting the resulting culture before substantial exponential growth has occurred. The process affords high yields of those products, low contamination of those products and ease in fermentation culture monitoring, handling and purification.

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