C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C12N 1/21 (2006.01) C12N 15/67 (2006.01)
Patent
CA 2211435
An amplifier sequence at least 90 % homologous with SEQ ID No. 1: TCTATAAATA X1X2X3GC Y1Y2Y3GG TATTTGGGGT TGGCAGCTGT T, wherein X1, X2 and X3, which are the same or different, may be independently C or G, C or G and C or A respectively; and Y1, Y2 and Y3, which are the same or different, may be independently T or C, C or G and T or C respectively, is disclosed. Said sequence may be included in an expression vector or sequence further containing a sequence coding for a protein and a promoter. Such sequences enable very strong amplification of the transcription of the gene coding for the protein.
Séquence amplificatrice présentant une homologie d'au moins 90 % avec la séquence SEQ ID No. 1 suivante: TCTATAAATA X1X2X3GC Y1Y2Y3GG TATTTGGGGT TGGCAGCTGT T dans laquelle: X1, X2 et X3 peuvent représenter indépendamment respectivement C ou G, C ou G, et C ou A; Y1, Y2 et Y3 peuvent représenter indépendamment respectivement T ou C, C ou G, et T ou C. Une telle séquence peut être comprise dans une séquence ou un vecteur d'expression contenant en outre une séquence codant pour une protéine et un promoteur. De telles séquences permettent d'amplifier très fortement la transcription du gène codant pour la protéine.
Li Zhen Lin
Paulin Denise
Institut Pasteur
Robic
Universite Paris 7
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