C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/48 (2006.01) C07K 19/00 (2006.01) C12N 9/12 (2006.01) C12N 15/00 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2533440
The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.
L'invention concerne un procédé d'amplification de l'ATP consistant à faire agir une protéine fusionnée kinase d'acide polyphosphorique/adénylate-kinase (PKK-ADK) sur un mélange contenant l'ATP, de l'AMP et un composé d'acide polyphosphorique afin d'amplifier et de détecter une très petite quantité d'ATP exogène. L'invention concerne également un procédé d'amplification de l'ATP ultrasensible permettant d'amplifier et de détecter l'ATP sur le niveau de chaque cellule et un procédé d'analyse microbienne ultrasensible basé sur ce procédé d'amplification de l'ATP.
Japan Science And Technology Agency
Kirby Eades Gale Baker
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