Amylase determination

Details Amylase determination Amylase determination

C - Chemistry, Metallurgy

12

Q

150/15.2

C12Q 1/40 (2006.01)

Patent

CA 1125634

AMYLASE DETERMINATION Abstract A method for the determination of amylase, based on the cleavage of colorimetric, ultraviolet absorbing or fluorometric substances, commonly referred to as chromogenic substances as p-nitrophenol derivatives of oligo- saccharides of chain length 4-10 glucose units. Oligosaccharides of this chain length are resistant to cleavage by .alpha. and/or .beta.-glycosidase and contain endo-.alpha.-1,4 linkages which are required for amylase activity. Cleavage of the .alpha.-1,4 bonds by amylase produces smaller fragments which are acted uponby .alpha.-glucosidage and/or .beta.-glucosidase to liberate a chromophore. The rate of appearance of the chromophore is proportional to amylase activity and lends itself to either rate or endpoint determinations. The qualitative or quantitative measurement of amylase concentrations in a sample is useful in medical diagnosis.

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