Amylolytic enzyme variants

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/28 (2006.01) A21D 8/04 (2006.01)

Patent

CA 2320813

The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

On a découvert de façon surprenante des similarités et des différences structurales entre la structure de Novamyl? et les structures d'origine de CgTases, et sur cette base, on a conçu des variantes d'alpha-amylase maltogène ayant une activité CGTase et des variantes de CGTase ayant une activité alpha-amylase maltogène. D'autre part, sur la base d'une homologie de séquence entre Novamyl? et CGTases, on a conçu des enzymes hybrides, une ou plusieurs améliorations ayant été apportées aux propriétés spécifiques des enzymes parents, par une méthodologie de l'ADN de recombinaison.

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