An assay for the measurement of dna synthesis rates

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2281111

Use of nonradioactive tracer technology used to measure DNA synthesis rates. DNA synthesis is determined by administering one or more stable isotope labelled precursors to one or more of DNA molecules and the amount of the stable isotope label in the DNA that is extracted from cells that are of interest is measured.

L'invention concerne l'utilisation d'une technique de marquage non radioactif pour mesurer les taux de synthèse de l'ADN. On détermine cette synthèse en administrant un ou plusieurs précurseurs marqués par des isotopes stables, à une ou plusieurs molécules d'ADN, et on mesure la dose du marqueur isotopique stable dans l'ADN extrait des cellules d'intérêt.

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