An efficient system for rna silencing

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01)

Patent

CA 2460686

The invention relates to a method for efficient RNA silencing of target genes in eucaryotic cells, particularly plant cells. Consequently, the method can be used to reduce the phenotypic expression of an endogenous gene in a plant cell. Furthermore the method can be applied in a high throughput screening for mutant phenotypes as a result of RNA silencing of any endogene.

L'invention concerne un procédé de blocage d'ARN de gènes cibles dans des cellules eucaryotes, en particulier des cellules végétales. Le procédé peut donc servir à réduire l'expression phénotypique d'un gène endogène dans une cellule végétale. En outre, le procédé peut être appliqué dans un criblage à haut rendement de phénotypes mutants à la suite du blocage d'ARN d'un endogène quelconque.

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