An electrochemical method for enrichment of microorganism, a...

G - Physics – 01 – N

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G01N 33/18 (2006.01)

Patent

CA 2378580

Disclosed herein is a biosensor that allows an organic substance concentration or BOD of a sample to be electrochemically measured in anaerobic condition using a mediator-less biofuel cell. The biosensor utilizes electrochemically active bacteria that were contained in wastewater and sluge and densely cultured during the operation procedure of the biofuel cell for the BOD measurement, as a microbial catalyst of the biofuel cell used in the biosensor. As a result, the biosensor can be operated without an artificial addition of microorganisms, and allows the microorganisms to be maintained at a suitable activity depending on the nature of wastewater. In addition, the biofuel cell used in the biosensor can be operated in a stable manner over six months or more.

L'invention porte sur un biodétecteur permettant de mesurer électrochimiquement dans des conditions anaérobie la concentration d'échantillons en substances organiques ou en DBO, à l'aide de cellules à carburant biologique sans médiateur. Ledit biodétecteur utilise des bactéries électrochimiquement actives présente dans les eaux usées et les boues, objets d'une culture dense pendant le processus d'exploitation de la cellule à carburant biologique en vue de la mesure du DBO, comme catalyseur microbien de la cellule biologique utilisée dans le biodétecteur. Il en résulte que le biodétecteur peut fonctionner sans adjonction artificielle de micro-organismes tout en permettant aux micro-organismes de maintenir une activité satisfaisante fonction de la nature des eaux usées. En outre la cellule biologique du biodétecteur peut fonctionner stablement pendant six mois ou plus.

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