An embedding method and apparatus for the preparation of...

G - Physics – 01 – N

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G01N 1/42 (2006.01) C12N 5/00 (2006.01) G01N 1/36 (2006.01)

Patent

CA 2621386

Various embodiments for an apparatus and method for preparing frozen tissue specimens are described. In an exemplary embodiment, the apparatus includes a sample container for receiving an excised tissue sample and embedding material. The sample container includes a flat base along which the tissue sample is flattened. The apparatus further includes a freezing box with a freezing platform and a freezing agent. The sample container is placed on the freezing platform to begin the freezing process. A chuck with a generally planar surface can be placed on the sample container during the freezing process joining the chuck, tissue specimen, and sample container base in generally parallel planes. In some cases, the sample container can be made from a suitable material, such as plastic, that is at least semi-transparent to allow visual confirmation of tissue flatness and freezing.

L~invention concerne divers modes de réalisation d~un appareil et d~un procédé de préparation de prélèvements de tissu congelés. Dans un exemple de mode de réalisation, l~appareil comporte un récipient à échantillon servant à recevoir un échantillon de tissu excisé et une matière d~enrobage. Le récipient à échantillon comporte une base plate le long de laquelle l~échantillon de tissu est aplati. L~appareil comporte en outre une boîte de congélation comportant une plate-forme de congélation et un agent de congélation. Le récipient à échantillon est placé sur la plate-forme de congélation au début du processus de congélation. Un mandrin présentant une surface généralement plane peut être placé sur le récipient à échantillon au cours du processus de congélation de manière à positionner le mandrin, le prélèvement de tissu et la base du récipient à échantillon dans des plans généralement parallèles. Dans certains cas, le récipient à échantillon peut être constitué d~une matière adéquate, telle que le plastique, qui est au moins semi-transparente de façon à permettre la confirmation visuelle de l~aplatissement et de la congélation du tissu.

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