An enzyme and enzyme preparation with endoglucanase activity

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) C11D 3/386 (2006.01) C12N 1/15 (2006.01) C12N 9/42 (2006.01) C12S 11/00 (2006.01) D21C 5/02 (2006.01) D21H 21/10 (2006.01)

Patent

CA 2201871

An enzyme composition with substantial cellulolytic, especially endoglucanase, activity at alkaline conditions which is derived from or producible by fungi from the genus Acremonium, provided that the enzyme composition is not immunologically cross-reactive with antibodies raised against endoglucanases (EC 3.2.1.4) from Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophila, CBS 117.65, or Cephalosporium sp., RYM-202. The invention further relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity. The enzyme composition and the isolated or cloned and expressed cellulase of the invention may be useful in any industrial process requiring an alkaline cellulase, e.g. for providing localised variation in the colour density of dyed fabric such as stone-washing of denim, for improving the drainage of an aqueous suspension of paper pulp, for the de-inking of recycled paper, in detergent compositions and in fabric softeners.

La présente invention concerne une composition enzymatique présentant, dans des conditions alcalines, une activité cellulolytique, en l'occurrence endoglucanase. L'enzyme est dérivée ou produite à partir d'un champignon du genre Acremonium dans la mesure où la composition ne présente pas de réactivité croisée avec des anticorps dirigés contre des endoglucanases (EC 3.2.1.4) des espèces Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophilia, CBS 117.65 ou Cephalosporium, RYM-202. L'invention concerne également un produit d'assemblage d'ADN comprenant une séquence d'ADN codant une enzyme à activité endoglucanase. La composition enzymatique et la cellulase isolée ou clonée et exprimée selon la présente invention sont utilisables dans tout processus industriel nécessitant une cellulase alcaline, par exemple, pour obtenir des variations localisées de la densité de couleurs (cas du lavage à la pierre du denim), pour améliorer le drainage d'une suspension aqueuse de la pâte à papier ou pour désencrer le papier de récupération, dans des compositions détergentes, et enfin dans des assouplissants pour textiles.

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