An enzyme feed additive and animal feed including it

Details An enzyme feed additive and animal feed including it An enzyme feed additive and animal feed including it

A - Human Necessities

23

K

A23K 1/165 (2006.01) C12N 9/42 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2156066

The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20 % by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20 % by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20 % by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, triticale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

L'invention concerne une composition utilisée comme additif alimentaire comprenant une ou plusieurs endoglucanases, et de 0 à 20 % en poids, basé sur la teneur en cellulase de la composition, d'une cellobiohydrolase. La cellulase peut renfermer une ou plusieurs endoglucanases telles que EGI, EGII, EGIII et tout dérivé fonctionnellement actif de n'importe laquelle de celles-ci. Ces endoglucanases peuvent être obtenues à partir d'une souche génétiquement modifiée du champignon <u>Trichoderma</u>. L'invention concerne également un additif alimentaire à base d'enzymes qui comprend une EGI et/ou EGII dépourvues du domaine de liaison de cellulose, et 0 à 20 % en poids, basé sur la teneur en cellulase de l'additif, d'une cellobiohydrolase. Un autre additif alimentaire à base d'enzymes comprend un véhicule à base de céréales, une ou plusieurs endoglucanases, et 0 à 20 % en poids, basé sur la teneur en cellulase de l'additif, d'une cellobiohydrolase. Ces additifs alimentaires à base d'enzymes peuvent être incorporés dans un produit alimentaire à base de céréales comprenant au moins une céréale choisie parmi l'orge, le blé, le triticale, le seigle et le maïs. L'additif alimentaire a l'avantage d'améliorer l'indice de conversion alimentaire et/ou de faciliter la digestibilité d'un produit alimentaire à base de céréales dans lequel il a été incorporé.

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