An enzyme with lipolytic activity

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/55 (2006.01) C11D 3/386 (2006.01) C12N 1/19 (2006.01) C12N 9/20 (2006.01)

Patent

CA 2203398

The invention relates, inter alia, to a DNA construct comprising a DNA sequence which encodes an enzyme exhibiting lipolytic activity and which comprises: a) the DNA sequence shown in SEQ ID No. 1, or b) an analogue of the DNA sequence shown in SEQ ID No. 1 which i) is homologous with the DNA sequence shown in SEQ ID No. 1, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 1, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 1, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified lipolytic enzyme which is encoded by the DNA sequence shown in SEQ ID No. 1 and/or which is derived from Humicola insolens DSM 1800. Also disclosed, inter alia, are: lipolytic enzymes encoded by such DNA constructs; and enzyme preparations, detergent additives and detergent compositions comprising such lipolytic enzymes.

L'invention concerne, entre autres, un ADN de recombinaison constitué d'une séquence nucléotidique codant pour une enzyme présentant une activité lipolytique et qui comprend: a) la séquence nucléotidique présentée dans le N~ ID SEQ 1, ou b) un analogue de la séquence nucléotidique présentée dans le N~ ID SEQ 1, lequel (i) est homologue de la séquence nucléotidique présentée dans le N~ ID SEQ 1, et/ou (ii) s'hybride avec la même sonde oligonucléotidique que celle à laquelle s'hybride la séquence nucléotidique du N~ ID SEQ 1, et/ou (iii) code un polypeptide qui réagit immunologiquement avec un anticorps dirigé contre une enzyme lipolytique purifiée codée par la séquence nucléotidique du N~ ID SEQ 1 et/ou qui est obtenu à partir de Humicola insolens DSM 1800. L'invention comprend également des enzymes lipolytiques codées par ces ADN de recombinaison, des préparations enzymatiques, des additifs pour détergents et des compositions détersives constituées de ces enzymes lipolytiques.

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