G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 15/02 (2006.01) G01N 30/74 (2006.01) G01N 21/53 (2006.01) G01N 27/26 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2072004
A high angle tight scattering detector using classical Rayleigh scattering. A high intensity arc light source, filtered to leave only one wavelength illuminates a flow cell. Through he flow cell, very small, particles such as biological proteins flow in solution after separation by HPLC or some other means. A UV detector generates data regarding the weight concentration of the eluting particles and a scattered tight detector collecting scattered light at angles of approximately 90° generates a scattered light signal. The incident light intensity is also measured. The average molecular weight is then computed using the scattered and incident light data, the weight concentration data and a simplified mathematical relationship from which the size factor P and the viral coefficients have been eliminated.
Cunico Robert L.
Dollinger Gavin D.
Kunitani Michael G.
Borden Ladner Gervais Llp
Cetus Corporation
Chiron Corporation
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