An iap binding peptide or polypeptide and methods of using...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 38/04 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2420534

An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a peptide or polypeptide of Smac having at least two contiguous amino acid residues derived from at least residues 56-139 of SEQ ID NO: 1 and of which up to 184 contiguous amino acid residues can be derived from residues 56-239 of SEQ ID NO: 1, a functional variant of each or a functional equivalent of each, each of which is capable of specifically binding to at least a portion of an Inhibitor of Apoptosis protein. This peptide can be used in a method to modulate apoptosis or to identify modulators of apoptosis as well as in therapeutic uses.

L'invention concerne une molécule d'acide nucléique isolée comprenant un polypeptide à séquence codant un peptide ou un polypeptide de Smac qui présente au moins deux résidus d'acides aminés contigus dérivés au moins des résidus 56-139 de SEQ ID NO: 1, sachant qu'il est possible de dériver jusqu'à 184 résidus d'acides aminés contigus à partir des résidus 56-239 de SEQ ID NO: 1. L'invention concerne également un variant fonctionnel ou un équivalent fonctionnel dans chacun des cas, chaque variant ou équivalent étant capable de liaison spécifique avec au moins une partie d'inhibiteur de protéine de l'apoptose. On peut utiliser ce type de peptide dans le cadre d'un procédé qui permet de moduler l'apoptose ou d'identifier des modulateurs de l'apoptose, et aussi à des fins thérapeutiques.

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