An improved drugscreening method

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/90 (2006.01) A01K 67/027 (2006.01) A61K 35/32 (2006.01) A61K 49/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2121582

2121582 9308301 PCTABS00021 The present invention relates to a method for determining the effect of a drug or a treatments, comprising introducing, in a non-human vertebrate recipient which may be an immunodeficient, e.g. a nude mouse, cells from a donor vertebrate, e.g. a human, which mediate the disease, the donor cell or cells of the recipient being modified, e.g. marked with a genetic marker such as lacZ gene, administering the drug or the treatment to the non-human recipient vertebrate, and determining the effect of the drug or the treatment. When the genetic marker is lacZ gene, the detection is performed by staining with the chromogenic substrate X-gal resulting in a blue staining of the labelled cell. In a further aspect, the invention relates to a method for controlling the progression of cancer cells in a human or animal patient, comprising administering to the patient fibroblasts which have been transformed with a genetic construct expressing a gene product inhibiting the progression of cancer cells.

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