An improved method for preparing dna from serum and plasma

Details An improved method for preparing dna from serum and plasma An improved method for preparing dna from serum and plasma

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2297547

Described herein are methods for extracting DNA from serum or plasma, comprising contacting serum or plasma with alkali to yield alkalinized serum or plasma, heating the alkalinized serum or plasma to a temperature ranging from about 100 to about 110°C for a time ranging from about 5 to about 20 minutes, centrifuging ,the heated alkalinized serum or plasma to yield a DNA-containing supernatant, allowing the heated alkalinized serum or plasma to cool to room temperature, or about 25 °C, and recovering the DNA-containing supernatant. Also disclosed are methods for detecting a DNA-containing microorganism in serum or plasma.

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