An improved nucleic acid purification method

Details An improved nucleic acid purification method An improved nucleic acid purification method

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 1/06 (2006.01)

Patent

CA 2694411

The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including Tween 20. Also disclosed are composition and kit for performing the modified method.

L'invention concerne un procédé modifié pour la séparation des acides nucléiques à partir de cellules, comprenant les étapes consistant à : générer une solution aqueuse contenant l'acide nucléique en lysant les cellules avec une solution de lyse comprenant du SDS et du sel; et séparer les acides nucléiques intéressants des autres composants cellulaires. L'amélioration comprend l'ajout d'un détergent non ionique dans la solution de lyse de telle sorte que le SDS ne précipite pas, et qu'aucun chauffage de la solution ne soit nécessaire avant une lyse cellulaire. Les détergents non ioniques préférés sont de la famille du composé de polysorbate, y compris le Tween 20. L'invention concerne également un composé et un kit pour mettre en uvre le procédé modifié.

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