An improved yeast interaction trap assay

Details An improved yeast interaction trap assay An improved yeast interaction trap assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 1/19 (2006.01) C12N 15/10 (2006.01) C12N 15/81 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2304367

The present invention provides an improved yeast interaction trap method and reagents for the detection of novel protein-protein interactions. The invention comprises a dual bait system which improves the accuracy of library screens with an immediate selection to eliminate false positives. The dual bait system of the present invention also allows for comparative, simultaneous assessment of interactions between two related members of a protein family or a wild-type versus mutated form of the same protein.

L'invention concerne un procédé amélioré d'essai à la levure par piège d'interaction ainsi que des réactifs pour la détection de nouvelles interactions protéine-protéine. L'invention concerne également un système à double appât qui permet d'améliorer la précision du criblage des bibliothèques sur la base d'une sélection immédiate des faux positifs en vue de leur élimination. Le système d'appât en question permet aussi d'effectuer une évaluation comparative simultanée des interactions entre deux membres apparentés d'une famille de protéines ou entre une forme sauvage et une forme mutante de la même protéine.

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