An in vitro system for determining formation of a.beta. amyloid

Details An in vitro system for determining formation of a.beta. amyloid An in vitro system for determining formation of a.beta. amyloid

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) G01N 1/38 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2205085

The invention relates to rapid methods for determining formation of A.beta. amyloid and screening compounds which inhibit formation of A.beta. amyloid in vitro, as well as kits for carrying out the presesnt methods. Such an agent used in vivo may prevent, ameliorate or reverse the symptoms of Alzheimer's disease and A.beta. amyloidotic disorders related to Alzheimer's disease, Down's syndrome, and Guamanian amyotrophic lateral sclerosis/Parkinson's dementia complex. The process described in this invention involves the rapid induction of A.beta. amyloid by a heavy metal cation capable of binding to a polypeptide comprising at least amino acids 6 to 28 of A.beta., such as zinc to form amyloid and determination of formation of tinctorial A amyloid. Moreover, a method of determining effectiveness of a candidate anti-amyloidotic agent for prevention or treatment A amyloidosis is described which uses cells cultures which express at least a human A.beta. peptide.

L'invention se rapporte à des procédés rapides de détermination de l'amyloïde A beta et de criblage de composés qui inhibent la formation de l'amyloïde A beta in vitro, ainsi qu'à des kits servant à réaliser ces procédés. Ce type d'agent utilisé in vivo peut prévenir, améliorer ou faire rétrocéder les symptômes de la maladie d'Alzheimer et des troubles amyloïdotiques Ab relatifs à la maladie d'Alzheimer, au syndrome de Down et au complexe sclérose latérale amyotrophique de Guamanian/maladie de Parkinson. Le procédé de l'invention consiste à induire rapidement l'amyloïde A beta à l'aide d'un cation métallique lourd, capable de se fixer à un polypeptide comprenant au moins 6 à 28 acides amino d'A beta , tel que le zinc, pour obtenir un amyloïde, et à déterminer la formation de l'amyloïde A beta tinctorial. L'inventioin se rapporte en outre à un procédé de détermination de l'efficacité d'un agent anti-amyloïdotique candidat en vue de prévenir ou traiter l'amyloïdose A beta . Ce procédé met en oeuvre des cultures cellulaires qui expriment au moins un peptide A beta humain.

Affiliated with

Also associated with

Rating

An in vitro system for determining formation of a.beta. amyloid does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with An in vitro system for determining formation of a.beta. amyloid, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and An in vitro system for determining formation of a.beta. amyloid will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2088889