An in vivo gene expression system

Details An in vivo gene expression system An in vivo gene expression system

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) C12N 15/11 (2006.01) C12N 15/81 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2185550

The present invention relates generally to an in vivo system for gene expression and, more particularly, to the use of the system to screen for molecules which are capable of inhibiting, reducing, altering or otherwise modulating the expression of a target nucleotide sequence or the activity of a gene product. The in vivo system of the present invention is particularly but not exclusively useful for screening for antisense, sense or ribozymes constructs or transdominant polypeptides, small peptides or other chemical compounds and which are capable of inhibiting, reducing, altering or otherwise modulating expression of target genes or target genetic sequences or the activity of target gene products of commercial importance such as in the medical, agricultural and industrial fields.

L'invention concerne, de façon générale, un système in vivo destiné à l'expression de gènes et, en particulier, l'utilisation de ce système pour trier des molécules pouvant inhiber, réduire, modifier ou bien moduler l'expression d'une séquence nucléotidique cible ou l'activité d'un produit génique. Ce système in vivo sert notamment, mais non exclusivement, à trier des produits d'assemblages antisens, sens ou à base de ribozymes ou des polypeptides transdominants, des petits peptides ou d'autres composés chimiques pouvant inhiber, réduire, modifier ou, de façon générale, moduler l'expression de gènes ou de séquences génétiques cibles, ou l'activité de produits géniques cibles qui présentent une importance commerciale, par exemple dans les domaines de la médecine, de l'agriculture, et de l'industrie.

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